中国国家流感中心检测报告

Examining report of the national influenza centre of China

Measure things: “it is amazed by "  Disinfectant

  Inspect the unit by ready samples: It is amazed by the scientific research institute of the smell in the market of Puyang of Henan Province

  Inspect the purpose by ready samples: " it is amazed by "  Result of killing to influenza virus of disinfectant

  Inspect time by ready samples: January 6, 2005

  Detection time: Come on the 10th of January, 2005 February 25, 2005

        Report time: February 28, 2005

Detection method: The degree with diluting one " it shy " disinfectant and 10- 1  10- 6 dilutes influenza virus of degree after 4 degrees Centigrade accord with 3 hours, inoculate it in MDCK cell, 37 degrees Centigrade is trained for 48 hours. Observe the situation of causing cell's pathological change of influenza virus from behind disinfectant function. Set up the virus thiazole antivirus positive contrasting and negative contrast of physiological saline antivirus at the same time in experiment. The virus will be collected to train the liquid 48 hours later, congeal and test influenza virus blood, congeal the result as the final result evaluation index with influenza virus blood.

Material: Fujian / 411/ 200 (H3N2), A/ of influenza virus, keep malicious kind for national influenza in the centre; the blood congeals and drips and spends 128. The virus thiazole buys from the pharmaceutical factory of Yang Zhou, in order to resist influenza virus medicine routinely; the cell trains and produces for Germany GBICO with the reagent; other experiment reagent disposes the method and consults the relevant contents of S.O.P. of national influenza centre.

Result: Having carried on the experiment 3 times altogether, the experimental result is as form shows each time

Form 1

Form 2

Form 3

ed finally: According to the above experimental result, prove " it is amazed by " the disinfectant is to result of killing with certain one of influenza virus ", and this kind of function and dosage present positive correlation.” it is amazed by " better if the original liquid of disinfectant is known as the air disinfectant result of use ". But at the same time we find in the course of experiment, the disinfectant has certain toxicity to MDCK cell.

中国疾病预防控制中心病毒病预防控制所 国家流感中心

The virosis of control centre of the prevention from suffering from the diseases of China is prevented and controlled one     National influenza centre

2005年3月11日        March 11 , 2005